| Distance from | | Distance from |
| Barrie | | Owen Sound |
| | | |
| 0.0 | Hwy 400 Interchange - Exit #98 (Barrie) | 116.1 |
| | | |
| 5.9 | Simcoe Road 27 Junction (Formerly Hwy 27 - To Elmvale) | 110.2 |
| | | |
| 14.6 | Simcoe Road 28 Junction (Minesing) | 101.5 |
| | | |
| 17.5 | Simcoe Road 22 Junction (To Anten Mills) | 98.6 |
| | | |
| 30.7 | Simcoe Road 10 Junction (To Angus) | 85.4 |
| | | |
| 37.8 | Simcoe Road 91 Junction (Formerly Hwy 91 - Stayner) | 78.3 |
| | | |
| 43.1 | Mosley Street Roundabout (Hwy 7149 - Wasaga Beach) | 73.0 |
| | | |
| 48.8 | Poplar Sideroad Roundabout (To Bowers Beach) | 67.3 |
| | | |
| 49.8 | Old Hwy 26 (Hwy 7148 - To Bowers Beach) | 66.3 |
| | | |
| 53.5 | Hurontario Street (Formerly Hwy 24 - Collingwood) | 62.6 |
| | | |
| 60.5 | Grey Road 21 Junction (To Blue Mountain) | 55.6 |
| | | |
| 63.3 | Grey Road 19 Junction (Craigleith) | 52.8 |
| | | |
| 70.4 | Grey Road 40 Junction (To Chatsworth) | 45.7 |
| | | |
| 73.0 | Grey Road 2 Junction (Thornbury) | 43.1 |
| | | |
| 76.9 | Grey Road 113 Junction (Thornbury) | 39.2 |
| | | |
| 86.2 | Grey Road 7 Junction (Meaford) | 29.9 |
| | | |
| 93.6 | Grey Road 112 Junction (To Grey Road 12) | 22.5 |
| | | |
| 100.5 | Grey Road 18 Junction (Woodford) | 15.6 |
| | | |
| 111.6 | Grey Road 15 Junction (Bothwell's Corner) | 4.5 |
| | | |
| 116.1 | Hwy 6, Hwy 10 & Hwy 21 Junction (Owen Sound) | 0.0 |
