| Distance from | | Distance from |
| Toronto | | Highway 69 |
| | | |
| 0.0 | Maple Leaf Drive Overpass (Start of Hwy 400) | 226.4 |
| | | |
| 0.4 | Exit #20 - Jane Street Interchange (Toronto) | 226.0 |
| | | |
| 1.6 | Exit #21 - Hwy 401 Interchange (Toronto) | 224.8 |
| | | |
| 6.0 | Exit #25 - Finch Avenue Interchange (Toronto) | 220.4 |
| | | |
| 8.1 | Exit #27 - Steeles Avenue Interchange (Toronto) | 218.3 |
| | | |
| 9.3 | Exit #26 - Hwy 407 ETR Interchange (Vaughan) | 217.1 |
| | | |
| 10.2 | Exit #29 - Hwy 7 Interchange (Vaughan) | 216.2 |
| | | |
| 12.3 | Exit #31 - Langstaff Road Interchange (Vaughan) | 214.1 |
| | | |
| 13.7 | Exit #32 - Bass Pro Mills Drive Interchange (Vaughan) | 212.7 |
| | | |
| 14.4 | Exit #33 - Rutherford Road Interchange (Vaughan) | 212.0 |
| | | |
| 16.5 | Exit #35 - Major Mackenzie Drive Interchange (Vaughan) | 209.9 |
| | | |
| 18.6 | Exit #37 - Teston Road Interchange (Vaughan) | 207.8 |
| | | |
| 24.9 | Exit #43 - King Road Interchange (To King City) | 201.5 |
| | | |
| 34.1 | Exit #52 - Lloydtown-Aurora Road Interchange (To Aurora) | 192.3 |
| | | |
| 37.3 | Exit #55 - Hwy 9 Interchange (To Newmarket) | 189.1 |
| | | |
| 40.2 | Exit #58 - Canal Road Interchange (To Bradford) | 186.2 |
| | | |
| 43.0 | Exit #61 - Line 5 Interchange (To Bradford) | 183.4 |
| | | |
| 45.8 | Exit #64 - Hwy 88 Interchange (To Bradford) | 180.6 |
| | | |
| 57.2 | Exit #75 - Hwy 89 Interchange (To Cookstown) | 169.2 |
| | | |
| 66.9 | Exit #85 - Innisfil Beach Road Interchange (To Thornton) | 159.5 |
| | | |
| 71.9 | Exit #90 - Mapleview Drive Interchange (Barrie) | 154.5 |
| | | |
| 75.7 | Exit #94 - Essa Road Interchange (Formerly Hwy 27 - Barrie) | 150.7 |
| | | |
| 78.1 | Exit #96 - Dunlop Street Interchange (Formerly Hwy 90 - Barrie) | 148.3 |
| | | |
| 80.5 | Exit #98 - Hwy 26 Interchange (Bayfield Street - Barrie) | 145.9 |
| | | |
| 83.2 | Exit #102 - Duckworth Street Interchange (Barrie) | 143.2 |
| | | |
| 85.9 | Exit #106 - Hwy 11 Interchange (To Orillia) | 140.5 |
| | | |
| 92.5 | Exit #111 - Forbes Road Interchange (To Midhurst) | 133.9 |
| | | |
| 98.8 | Exit #117 - Horseshoe Valley Road Interchange (To Craighurst) | 127.6 |
| | | |
| 102.3 | Exit #121 - Hwy 93 Interchange (To Midland) | 124.1 |
| | | |
| 112.7 | Exit #131 - Mount St. Louis Road Interchange (Mount St. Louis-Moonstone Resort) | 113.7 |
| | | |
| 117.6 | Exit #136 - Moonstone Road Interchange (To Moonstone) | 108.8 |
| | | |
| 120.2 | Exit #137 - Hwy 7174 Interchange (Lower Big Chute Road - To Coldwater) | 106.2 |
| | | |
| 123.0 | Exit #141 - Hwy 12 East Interchange (To Orillia) | 103.4 |
| | | |
| 128.9 | Exit #147 - Hwy 12 West Interchange (To Midland) | 97.5 |
| | | |
| 131.2 | Exit #149 - Quarry Road Interchange (To Waubaushene) | 95.2 |
| | | |
| 135.2 | Exit #153 - Port Severn Road Interchange (To Port Severn) | 91.2 |
| | | |
| 137.3 | Exit #156 - Muskoka District Road 5 Interchange (To Honey Harbour) | 89.1 |
| | | |
| 143.6 | Exit #162 - Muskoka District Roads 34 & 48 Interchange (To Big Chute) | 82.8 |
| | | |
| 150.9 | Exit #168 - Georgian Bay Road Interchange (To Wood Landing) | 75.5 |
| | | |
| 156.4 | Exit #174 - Muskoka District Road 33 Interchange (To Gibson Lake) | 70.0 |
| | | |
| 159.5 | Exit #177 - Muskoka District Roads 32 & 38 Interchange (To Bala) | 66.9 |
| | | |
| 164.0 | Exit #182 - Iroquois Cranberry Growers Drive Interchange (To Wahta First Nation) | 62.3 |
| | | |
| 167.2 | Exit #185 - Muskoka District Road 12 Interchange (To Twelve Mile Bay) | 59.2 |
| | | |
| 171.2 | Exit #189 - Hwy 7289 Interchange (Lake Joseph Road - To MacTier) | 55.2 |
| | | |
| 179.7 | Healey Lake Road Overpass | 46.7 |
| | | |
| 189.7 | Exit #207 - Hwy 141 Interchange (To Huntsville) | 36.7 |
| | | |
| 195.6 | Exit #213 - Hwy 7290 Interchange (Lake Joseph Road - To Horseshoe Lake) | 30.8 |
| | | |
| 198.7 | Exit #214 - Seguin Trail Interchange (To Horseshoe Lake) | 27.7 |
| | | |
| 201.9 | Exit #217 - Oastler Park Drive Interchange (To Oastler Lake) | 24.5 |
| | | |
| 205.3 | Exit #220 - Hwy 518 Interchange (To Orrville) | 21.1 |
| | | |
| 208.9 | Exit #224 - Bowes Street Interchange (Parry Sound) | 17.5 |
| | | |
| 211.4 | Exit #229 - Parry Sound Drive Interchange (Parry Sound) | 15.0 |
| | | |
| 214.0 | Exit #231 - Hwy 124 Interchange (Parry Sound) | 12.4 |
| | | |
| 219.2 | Exit #236 - Hwy 7910 Interchange (Avro Arrow Road - Nobel) | 7.2 |
| | | |
| 224.6 | Exit #241 - Hwy 559 Interchange (To Killbear Provincial Park) | 1.8 |
| | | |
| 226.4 | Hwy 69 - End of 4-lane Highway (1.8 km North of Hwy 559 Interchange) | 0.0 |
