| Distance from | | Distance from |
| Blue Water Bridge | | London |
| | | |
| 0.0 | Canada Customs Blue Water Bridge (Start of Hwy 402) | 102.5 |
| | | |
| 0.7 | Exit #1 - Front Street Interchange (Sarnia) | 101.8 |
| | | |
| 1.1 | Exit #2 - Christina Street Interchange (Sarnia) | 101.4 |
| | | |
| 3.0 | Exit #3 - Indian Road Interchange (Sarnia) | 99.5 |
| | | |
| 5.4 | Exit #6 - Hwy 40 & Modeland Road Interchange (Sarnia) | 97.1 |
| | | |
| 8.2 | Exit #9 - Airport Road Interchange (Sarnia) | 94.3 |
| | | |
| 14.7 | Exit #15 - Lambton Road 26 Interchange (To Brights Grove) | 87.8 |
| | | |
| 24.0 | Exit #25 - Lambton Road 21 Interchange (Formerly Hwy 21 South - To Petrolia) | 78.5 |
| | | |
| 33.3 | Exit #34 - Hwy 21 North & Lambton Road 8 Interchange (To Forest) | 69.2 |
| | | |
| 37.0 | Lambton Road 22 (Formerly Hwy 7) Overpass | 65.5 |
| | | |
| 43.5 | Exit #44 - Hwy 79 Interchange (To Watford) | 59.0 |
| | | |
| 50.9 | Middlesex-Lambton County Boundary | 51.6 |
| | | |
| 54.6 | Exit #56 - Middlesex Road 6 Interchange (To Kerwood) | 47.9 |
| | | |
| 63.8 | Exit #65 - Hwy 81 Interchange (To Strathroy) | 38.7 |
| | | |
| 68.1 | Exit #69 - Middlesex Road 39 Interchange (To Strathroy) | 34.4 |
| | | |
| 80.8 | Exit #82 - Middlesex Road 14 Interchange (To Mount Brydges) | 21.7 |
| | | |
| 84.3 | Exit #86 - Hwy 2 Interchange (To Delaware) | 18.2 |
| | | |
| 96.6 | Exit #98 - Hwy 4 Interchange (To Lambeth) | 5.9 |
| | | |
| 98.8 | Exit #100 - Wonderland Road Interchange (To London) | 3.7 |
| | | |
| 102.5 | Hwy 401 Interchange - End of Hwy 402 (To London) | 0.0 |
