| Distance from | | Distance from |
| Woodstock | | Mississauga |
| | | |
| 0.0 | Highway 401 Interchange (Woodstock - Start of Highway 403) | 125.4 |
| | | |
| 5.4 | Exit #5 - Oxford Road 55 Interchange (Formerly Highway 53 - To Eastwood) | 120.0 |
| | | |
| 15.3 | Exit #15 - Brant Road 25 Interchange (To Princeton) | 110.1 |
| | | |
| 26.7 | Exit #27 - Highway 24 South Interchange (To Simcoe) | 98.7 |
| | | |
| 30.2 | Exit #30 - Oak Park Road Interchange (Brantford) | 95.2 |
| | | |
| 33.1 | Exit #33 - Paris Road Interchange (Formerly Highway 2 - Brantford) | 92.3 |
| | | |
| 35.7 | Exit #36 - Highway 24 North Interchange (King George Road - Brantford) | 89.7 |
| | | |
| 38.3 | Exit #38 - Wayne Gretzky Parkway Interchange (Brantford) | 87.1 |
| | | |
| 40.7 | Exit #41 - Garden Avenue Interchange (To Cainsville) | 84.7 |
| | | |
| 55.2 | Exit #55 - Hwy 52 Interchange (To Copetown) | 70.2 |
| | | |
| 58.5 | Exit #58 - Wilson Street Interchange (Formerly Highway 2 - Ancaster) | 66.9 |
| | | |
| 60.3 | Exit #60 - Fiddler's Green Road Interchange (Ancaster) | 65.1 |
| | | |
| 61.4 | Exit #61 - Highway 6 South Interchange (To Caledonia) | 64.0 |
| | | |
| 64.3 | Exit #64 - Lincoln Alexander Parkway Interchange (Hamilton) | 61.1 |
| | | |
| 68.9 | Exit #69 - Aberdeen Avenue Interchange (Hamilton) | 56.5 |
| | | |
| 70.3 | Exit #70 - Main Street Interchange (Formerly Highway 2 & Highway 8 - Hamilton) | 55.1 |
| | | |
| 72.5 | Exit #73 - York Boulevard Interchange (Hamilton) | 52.9 |
| | | |
| 73.9 | Exit #74 - Highway 6 North Interchange (To Guelph) | 51.5 |
| | | |
| 77.3 | Exit #78 - Waterdown Road Interchange (To Waterdown) | 48.1 |
| | | |
| 79.6 | Exit #80 - Highway 407 Express Toll Route Interchange (To Milton) | 45.8 |
| | | |
| 81.7 | QEW Exit #100 - Queen Elizabeth Way Interchange (To Niagara) | 43.7 |
| | | |
| 82.5 | QEW Exit #101 - Brant Street Interchange (Burlington) | 42.9 |
| | | |
| 84.4 | QEW Exit #102 - Guelph Line Interchange (Burlington) | 41.0 |
| | | |
| 86.5 | QEW Exit #105 - Walkers Line Interchange (Burlington) | 38.9 |
| | | |
| 88.5 | QEW Exit #107 - Appleby Line Interchange (Burlington) | 36.9 |
| | | |
| 90.5 | QEW Exit #109 - Burloak Drive Interchange (Burlington) | 34.9 |
| | | |
| 92.7 | QEW Exit #111 - Bronte Road Interchange (Formerly Highway 25 - Oakville) | 32.7 |
| | | |
| 94.8 | QEW Exit #113 - Third Line Interchange (Oakville) | 30.6 |
| | | |
| 97.9 | QEW Exit #116 - Dorval Drive Interchange (Oakville) | 27.5 |
| | | |
| 100.0 | QEW Exit #118 - Trafalgar Road Interchange (Oakville) | 25.4 |
| | | |
| 101.5 | QEW Exit #119 - Royal Windsor Drive Interchange (Oakville) | 23.9 |
| | | |
| 104.6 | QEW Exit #123 - Queen Elizabeth Way Interchange (To Toronto) | 20.8 |
| | | |
| 104.7 | Exit #104 - Upper Middle Road Interchange (Mississauga) | 20.7 |
| | | |
| 106.6 | Exit #106 - Dundas Street Interchange (Formerly Highway 5 - Mississauga) | 18.8 |
| | | |
| 109.6 | Exit #109 - Highway 407 Express Toll Route Interchange (Mississauga) | 15.8 |
| | | |
| 111.7 | Exit #111 - Winston Churchill Boulevard Interchange (Mississauga) | 13.7 |
| | | |
| 113.3 | Exit #112 - Erin Mills Parkway Interchange (Mississauga) | 12.1 |
| | | |
| 117.9 | Exit #117 - Mavis Road Interchange (Mississauga) | 7.5 |
| | | |
| 120.0 | Exit #119 - Hurontario Street Interchange (Formerly Highway 10 - Mississauga) | 5.4 |
| | | |
| 122.6 | Exit #122 - Eglinton Avenue Interchange (Mississauga) | 2.8 |
| | | |
| 124.0 | Ramps to Highway 410 (To Brampton) | 1.4 |
| | | |
| 125.4 | Highway 401 Interchange (To Toronto) - End of Highway 403 | 0.0 |
