| Distance from | | Distance from |
| Queen Elizabeth Way | | Welland |
| | | |
| 0.0 | Queen Elizabeth Way Interchange (Start of Highway 406) | 25.0 |
| | | |
| 3.7 | Exit #4 - Fourth Avenue Interchange | 21.3 |
| | | |
| 5.8 | Exits #6 & 6A - Westchester Avenue & Geneva Street Interchange | 19.2 |
| | | |
| 8.7 | Exit #9 - Glendale Avenue Interchange | 16.3 |
| | | |
| 10.8 | Exits #10 & 11 - Highway 58 & St. David's Road Interchange | 14.2 |
| | | |
| 13.3 | Exit #14 - Beaverdams Road Interchange | 11.7 |
| | | |
| 16.4 | Exit #17 - Highway 20 Interchange | 8.6 |
| | | |
| 20.2 | Exit #21 - Merritt Road Interchange | 4.8 |
| | | |
| 22.3 | Exit #23 - Highway 7236 (Woodlawn Road) | 2.7 |
| | | |
| 25.0 | Highway 7146 (East Main Street - End of Highway 406) | 0.0 |
