| Distance from | | Distance from |
| Highway 401 | | Highway 10 |
| | | |
| 0.0 | Highway 401 Interchange (Start of Highway 410) | 22.1 |
| | | |
| 2.1 | Exit #2 - Courtney Park Drive Interchange | 20.0 |
| | | |
| 3.6 | Exit #3 - Peel Road 5 (Derry Road) Interchange | 18.5 |
| | | |
| 5.1 | Exit #5 - Highway 407 ETR Interchange | 17.0 |
| | | |
| 7.2 | Exit #7 - Peel Road 15 (Steeles Avenue) Interchange | 14.9 |
| | | |
| 9.6 | Exit #9 - Clark Boulevard Interchange | 12.5 |
| | | |
| 10.3 | Exit #10 - Queen Street Interchange (Formerly Highway 7 East) | 11.8 |
| | | |
| 11.8 | Exit #12 - Williams Parkway Interchange | 10.3 |
| | | |
| 13.3 | Exit #13 - Bovaird Drive Interchange (Formerly Highway 7 West) | 8.8 |
| | | |
| 15.1 | Exit #15 - Sandalwood Parkway Interchange | 7.0 |
| | | |
| 17.8 | Exit #18 - Mayfield Road Interchange | 4.3 |
| | | |
| 21.5 | Exit #21 - Hurontario Street Interchange | 0.6 |
| | | |
| 22.1 | Highway 10 - End of Highway 410 | 0.0 |
