| Distance from | | Distance from |
| Napanee | | Pembroke |
| | | |
| 0.0 | Highway 2 Junction (Napanee) | 206.0 |
| | | |
| 2.3 | Highway 401 Interchange (To Kingston) | 203.7 |
| | | |
| 20.1 | Lennox & Addington Road 3 Junction (To Marlbank) | 185.9 |
| | | |
| 28.7 | Lennox & Addington Road 13 Junction (Erinsville) | 177.3 |
| | | |
| 50.9 | Highway 7 Junction (Kaladar) | 155.1 |
| | | |
| 69.2 | Highway 506 Junction (To Plevna) | 136.8 |
| | | |
| 81.1 | Bon Echo Provincial Park Entrance | 124.9 |
| | | |
| 114.0 | Highway 28 Junction (Denbigh) | 92.0 |
| | | |
| 129.7 | Madawaska River Bridge (Griffith) | 76.3 |
| | | |
| 151.4 | Highway 132 Junction (To Renfrew) | 54.6 |
| | | |
| 171.3 | Highway 512 Junction (To Foymount) | 34.7 |
| | | |
| 172.4 | Highway 60 East Junction (Eganville) | 33.6 |
| | | |
| 176.2 | Highway 60 West Junction (To Barry's Bay) | 29.8 |
| | | |
| 186.2 | Renfrew Road 13 Junction (To Micksburg) | 19.8 |
| | | |
| 205.0 | Highway 17 Junction (To North Bay) | 1.0 |
| | | |
| 206.0 | Renfrew Road 19 Junction (Pembroke) | 0.0 |
