| Distance from | | Distance from |
| Browns Line | | Highway 7 |
| | | |
| 0.0 | Evans Avenue & Browns Line Interchange (Start of Highway 427) | 21.1 |
| | | |
| 0.5 | Queen Elizabeth Way Interchange | 20.6 |
| | | |
| 0.8 | The Queensway Interchange | 20.3 |
| | | |
| 2.4 | Highway 5 (Dundas Street) Interchange | 18.7 |
| | | |
| 4.3 | Burnhamthorpe Road Interchange | 16.8 |
| | | |
| 5.4 | Rathburn Road Interchange | 15.7 |
| | | |
| 7.0 | Ramps to Highway 27 and Highway 401 | 14.1 |
| | | |
| 8.0 | Highway 401 Overpass | 13.1 |
| | | |
| 11.0 | Dixon Road & Airport Road Interchange | 10.1 |
| | | |
| 12.4 | Highway 409 Interchange | 8.7 |
| | | |
| 15.0 | Rexdale Boulevard & Derry Road Interchange | 6.1 |
| | | |
| 16.8 | Finch Avenue Interchange | 4.3 |
| | | |
| 19.2 | Highway 407 (ETR) Interchange | 1.9 |
| | | |
| 21.1 | Highway 7 Interchange (End of Highway 427) | 0.0 |
