| Distance from | | Distance from |
| Browns Line | | Major Mackenzie Dr. |
| | | |
| 0.0 | Evans Avenue & Browns Line Interchange (Start of Highway 427) | 26.1 |
| | | |
| 0.3 | Queen Elizabeth Way Interchange | 25.8 |
| | | |
| 0.6 | The Queensway Interchange | 25.5 |
| | | |
| 2.1 | Highway 5 (Dundas Street) Interchange | 24.0 |
| | | |
| 4.0 | Burnhamthorpe Road Interchange | 22.1 |
| | | |
| 5.1 | Rathburn Road Interchange | 21.0 |
| | | |
| 6.8 | Ramps to Highway 27 and Highway 401 | 19.3 |
| | | |
| 7.6 | Highway 401 Overpass | 18.5 |
| | | |
| 10.1 | Dixon Road & Airport Road Interchange | 16.0 |
| | | |
| 11.4 | Highway 409 Interchange (Exit #13) | 14.7 |
| | | |
| 14.0 | Rexdale Boulevard & Derry Road Interchange (Exit #15) | 12.1 |
| | | |
| 15.8 | Finch Avenue Interchange (Exit #17) | 10.3 |
| | | |
| 18.1 | Highway 407 (ETR) Interchange (Exit #19) | 8.0 |
| | | |
| 19.7 | Highway 7 Interchange (Exit #21) | 6.4 |
| | | |
| 21.8 | Langstaff Road Interchange (Exit #23) | 4.3 |
| | | |
| 23.9 | Rutherford Road Interchange (Exit #25) | 2.2 |
| | | |
| 26.1 | Major Mackenzie Drive Interchange (End of Highway 427) | 0.0 |
