| Distance from | | Distance from |
| Long Point | | Shakespeare |
| | | |
| 0.0 | Long Point Provincial Park (Start of Highway 59) | 122.8 |
| | | |
| 8.0 | Norfolk Road 42 Junction (To Port Rowan) | 114.8 |
| | | |
| 16.4 | Highway 24 Junction (To Simcoe) | 106.4 |
| | | |
| 24.6 | Norfolk Road 1 Junction (To Simcoe) | 98.2 |
| | | |
| 39.1 | Highway 3 West Junction (Courtland) | 83.7 |
| | | |
| 50.4 | Highway 3 East Junction (Delhi) | 72.4 |
| | | |
| 67.4 | Oxford Road 18 Junction (Norwich) | 55.4 |
| | | |
| 72.4 | Oxford Road 14 Junction (To Beaconsfield) | 50.4 |
| | | |
| 80.0 | Oxford Road 13 Junction (To Courtland) | 42.8 |
| | | |
| 90.6 | Highway 401 Junction (Woodstock) | 32.2 |
| | | |
| 93.2 | Highway 2 East Junction (Woodstock) | 29.6 |
| | | |
| 94.4 | Highway 2 West Junction (To London) | 28.4 |
| | | |
| 107.1 | Oxford Road 8 Junction (Hickson) | 15.7 |
| | | |
| 117.1 | Oxford Road 24 Junction (Tavistock) | 5.7 |
| | | |
| 120.4 | Perth Road 33 Junction (To Stratford) | 2.4 |
| | | |
| 122.8 | Highway 7 & Highway 8 Junction (Shakespeare) | 0.0 |
