| Distance from | | Distance from |
| Kitchener | | Highway 21 |
| | | |
| 0.0 | Highway 7 Interchange (Kitchener) | 125.3 |
| | | |
| 0.3 | Wellington Street Interchange (Kitchener) | 125.0 |
| | | |
| 1.8 | Lancaster Street Interchange (Kitchener) | 123.5 |
| | | |
| 2.7 | Bridgeport Road Interchange (Waterloo) | 122.6 |
| | | |
| 4.2 | University Avenue Interchange (Waterloo) | 121.1 |
| | | |
| 6.6 | King Street Interchange (Waterloo) | 118.7 |
| | | |
| 7.9 | Northfield Drive Interchange (Waterloo) | 117.4 |
| | | |
| 13.1 | Waterloo Road 17 Junction (To St. Jacobs) | 112.2 |
| | | |
| 17.0 | Waterloo Road 21 Junction (To Elmira) | 108.3 |
| | | |
| 22.2 | Waterloo Road 86 Junction (To Guelph) | 103.1 |
| | | |
| 30.0 | Waterloo Road 5 Junction (Macton) | 95.3 |
| | | |
| 41.1 | Wellington Road 10 Junction (To Moorefield) | 84.2 |
| | | |
| 42.9 | Highway 19 Junction (To Milverton) | 82.4 |
| | | |
| 54.0 | Highway 23 North Junction (Listowel) | 71.3 |
| | | |
| 55.1 | Highway 23 South Junction (To Mitchell) | 70.2 |
| | | |
| 67.2 | Huron Road 34 Junction (To Gorrie) | 58.1 |
| | | |
| 75.2 | Huron Road 12 Junction (To Wroxeter) | 50.1 |
| | | |
| 81.6 | Highway 87 Junction (Bluevale) | 43.7 |
| | | |
| 88.7 | Highway 4 Junction (Wingham) | 36.6 |
| | | |
| 106.4 | Huron Road 1 Junction (Lucknow) | 18.9 |
| | | |
| 117.0 | Huron Road 7 Junction (To Ripley) | 8.3 |
| | | |
| 125.3 | Highway 21 Junction (Amberley) | 0.0 |
