| Distance from | | Distance from |
| Harriston | | Fennell |
| | | |
| 0.0 | Highway 9 & Highway 23 Junction (Harriston) | 112.4 |
| | | |
| 16.8 | Highway 6 Junction (Mount Forest) | 95.6 |
| | | |
| 30.2 | Wellington & Grey Road 14 Junction (Conn) | 82.2 |
| | | |
| 48.5 | Highway 25 Junction (To Grand Valley) | 63.9 |
| | | |
| 60.9 | Highway 10 North Junction (Shelburne) | 51.5 |
| | | |
| 62.2 | Dufferin Road 124 Junction (Formerly Highway 24 North - To Collingwood) | 50.2 |
| | | |
| 66.2 | Highway 10 & Highway 24 South Junction (Primrose) | 46.2 |
| | | |
| 75.7 | Dufferin Road 18 Junction (Airport Road) | 36.7 |
| | | |
| 85.1 | Highway 50 Junction (To Bolton) | 27.3 |
| | | |
| 91.4 | Simcoe Road 10 South Junction (Alliston) | 21.0 |
| | | |
| 92.4 | Simcoe Road 10 North Junction (To Angus) | 20.0 |
| | | |
| 103.1 | Highway 27 Junction (Cookstown) | 9.3 |
| | | |
| 107.0 | Highway 400 Interchange (To Toronto) | 5.4 |
| | | |
| 112.4 | Highway 11 Junction (Fennell) | 0.0 |
