| Distance from | | Distance from |
| Elfrida | | Eastwood |
| | | |
| 0.0 | Highway 20 & Highway 53 Junction (Elfrida) | 76.5 |
| | | |
| 10.1 | Upper James Street (Hamilton) | 66.4 |
| | | |
| 21.2 | Highway 2 East Junction (Ancaster) | 55.3 |
| | | |
| 22.8 | Highway 52 Junction (To Copetown) | 53.7 |
| | | |
| 36.1 | Highway 54 Junction (Cainsville) | 40.4 |
| | | |
| 39.7 | Park Road (Wayne Gretzky Parkway) | 36.8 |
| | | |
| 42.4 | Highway 2 West Junction (Brantford) | 34.1 |
| | | |
| 50.1 | Highway 24 Junction (To Simcoe) | 26.4 |
| | | |
| 54.3 | Brant Road 16 Junction (Bishopsgate Road) | 22.2 |
| | | |
| 73.1 | Highway 403 Interchange (To Woodstock) | 3.4 |
| | | |
| 76.5 | Highway 2 Junction (Eastwood) | 0.0 |
